CARDIOPROTECTIVE EFFECT OF METHANOLIC EXTRACT OF MAGNIFERA INDICA (MANGO) SEED FLOUR ON ACETAMINOPHEN-INDUCED CARDIOTOXICITY IN ALBINO RATS

Abstract

This study evaluated the effect of methanol extract of mango (Magnifera indica) seed flour on acetaminophen-induced cardiotoxicity in albino rats. Twenty four (24) male and female albino rats were divided into 6 groups consisted of 4 rats: a)Group1: Control group (no induction and treated with distilled water only);b) Group 2 (acetaminophen-induced and untreated); c) Group 3 (acetaminophen-induced and pre-treated with 1.2mg/kg b.w Aspirin); Groups 4, 5 and 6 (acetaminophen-induced and pre-treated with 200, 400 and 800mg/kg b.w of M. indica extract respevtively). Acetaminophen induced toxicities were applied to rats in groups 2-6 (2g/kg body weight of acetaminophen) on day 7.Phytochemical analyses of the extract, biochemical parameters; lactate dehydrogenase, creatinine kinase, blood glucose, AST, ALP, ALT, bilirubin, total protein, albumin and globulin concentrations were determined using standard methods. Phytochemical analysis revealed the presence of alkaloids, saponins, hydrogen cyanide and tannins. The LDH concentration of rats in the groups 4, 5 and 6 showed a significant (p<0.05) decrease compared to group 2. There was also a decrease in concentration of CK-MB in groups 4, 5 and 6 compared with the untreated group 2. A significant increase (p<0.05) was observed in blood glucose levels of rats in group 1 compared with rats in group 4 and 6.  A significant (p<0.05) decrease was observed in ALT and bilirubin levels of rats in groups 5 and 6 compared with those in group 2. Furthermore, a significant (p < 0.05) increase was observed in total protein and albumin levels in groups 4 and 5 compared with those in group 2. The results of this study therefore suggest that the methanol extract of mango seed flour may possess cardioprotective potentials on acetaminophen-induced cardiotoxicity in albino rats.