A descriptive cross sectional study was carried out among 150 nursing students at Nursing College of Khwaja Yunus Ali, Enayetpur, Sirajgonj, Bangladesh. The objective of the present study was to assess the knowledge and practice about breast self- examination of nursing students. The study found that the knowledge of the respondents on the overall breast self- examination are not complete knowledge. Majority respondents (90.0%) knew the method of breast self- examination. Among them (57.0%) had their complete knowledge, (30.0%) had their partial knowledge and (12.6%) had their wrong knowledge. About (90.0%) respondent’s had their practice of breast self-examination. Among them (11.11%) respondents examined daily, (18.52%) examined every week, (48.15%) examined once a month and (22.22%) had their irregular breast self -examination practice. About (78%) examined their breast during bathing and (22%) examined at night. It was revealed that most of them (48.0%) belonged to the age 22 years and the mean age of the students was 21.73 ± 1.028 years. About (70.0%) students come from nuclear family and (30.0%) students belonged to the extended family. This study though a little one, might provide important information to the concerned authority to take proper measures and also provide clear for farther in-depth study. The accessibility of health education and the counseling service to the students should be ensured so as to achieve the expected level of awareness on personal health care.