Urinary tract infection is one of the most common ailments in medical practice affecting people of all ages from neonate to geriatric age group. UTI is due to an inflammatory response of urothelium to the invading pathogenic organisms. To Assess the Culture and Sensitivity Pattern of Escherichia coli in Urinary Tract Infections. This cross sectional study was carried out in Dhaka Medical College Hospital, Dhaka, Bangladesh during the period of July 2011 to June 2013. Sampling technique was purposive and sample size was 70. Data were collected by interview of the patients, clinical examinations and laboratory investigations using the research instrument. The culture method used was disc diffusion method. The antibiotics tested for sensitivity were amikacin, gentamycin, ciprofloxacin, levofloxacin, cotrimoxazole, ceftriaxone, ceftazidime, cefixime and nitrofurantoin. The demographic data and the degree of sensitivity to antibiotics, whether sensitive or resistant, were recorded. The data collected for the period of one year were analyzed. Descriptive statistics was used to analyze the sensitivity of E. coli. Data were processed and analyzed using software SPSS (Statistical Package for Social Sciences) version 11.5. Incidence of bacteriuria and urosepsis were measured according to urine and blood culture report. Sensitivity pattern was also observed. The antibiotics tested for sensitivity were amikacin, gentamycin, ciprofloxacin, levofloxacin, cotrimoxazole, nitrofurantoin, ceftazidime, ceftriaxone and cefixime. The sensitivity pattern of E.coli to antibiotics in UTI was Amikacin and Ceftazidime. 66.7% 10 Celepime and Gentamycin 58.3% to Cotrimoxazole Ceftriaxone Cephradine and cefixime was last sensitive (16.7% and 83% respectively). E. coli in UTI was resistant to cefixime in 91.7%, to ciprofloxacin in 100.0% cases and to cotrimoxazole in 41.7% of cases. The study highlighted the marked resistance of E. coli to quinolones and third generation cephalosporins. Proper guidelines, supervision of antibiotic usage and constant information to the medical practitioners regarding the sensitivity pattern can help to prevent drug resistance.